Method for sensitive detection of multiple biological analytes

Details Method for sensitive detection of multiple biological analytes Method for sensitive detection of multiple biological analytes

2007-02-27

2007-02-27

Le, Long V. (Department: 1641)

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

C436S524000, C436S527000, C436S544000, C436S824000, C435S007100, C435S007910, C435S007930, C568S362000, C530S812000, C106S160100

Reexamination Certificate

active

10989072

ABSTRACT:
The present invention relates to a method of detecting biological analytes comprising suspending a target analyte in a suspending solution containing polymeric particles marked with a probe, wherein the probe has an affinity for said target analyte; adding recognition unit-peroxidase conjugate marker to the suspending solution; forming a complex of the target analyte, the polymeric particles marked with a probe, and the recognition unit-peroxidase conjugate marker; contacting a gelatin surface with the suspending solution; adding developer to the suspending solution in contact with the gelatin surface in the presence of phenol to attach the complex to the gelatin surface; washing the gelatin surface; and detecting the complex attached to the gelatin surface.

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