Method for selectively staining chitin-containing organisms

Details Method for selectively staining chitin-containing organisms Method for selectively staining chitin-containing organisms

2006-10-02

2010-06-29

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S034000, C435S243000, C435S040500, C435S040510

Reexamination Certificate

active

07745168

ABSTRACT:
The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the composition of these organisms. With the method of the present invention a sample to be analyzed is treated with a solution of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examined with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia.

REFERENCES:
patent: 7157242 (2007-01-01), Dao

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