Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-11-07
2006-11-07
Whisenant, Ethan (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S023100, C536S024300
Reexamination Certificate
active
07132241
ABSTRACT:
The present invention provides a method for selectively separating live cells which have expressed a specific mRNA comprising: a first step of introducing a marker which label mRNA into cells in a live cell group containing live cells which have expressed a specific mRNA; a second step of labeling said mRNA with said marker to obtain a live cell group containing live cells having the labeled mRNA; and a third step of detecting said labeled mRNA to identify the live cells having the labeled mRNA and separating the identified live cells selectively from said live cell group obtained in said second step.
REFERENCES:
patent: 4996143 (1991-02-01), Heller et al.
patent: 5225326 (1993-07-01), Bresser et al.
patent: 5629147 (1997-05-01), Asgari et al.
patent: 5728527 (1998-03-01), Singer et al.
patent: 5776782 (1998-07-01), Tsuji
patent: 5985549 (1999-11-01), Singer et al.
patent: 6127120 (2000-10-01), Graham et al.
patent: 6228592 (2001-05-01), Tsuji et al.
patent: 6562343 (2003-05-01), Levinson
patent: 6872525 (2005-03-01), Ishibashi et al.
patent: 11-285386 (1999-10-01), None
patent: WO93/23570 (1993-11-01), None
patent: WO98/13524 (1998-04-01), None
patent: WO98/33897 (1998-08-01), None
JP 130793/2000 Notice of Rejection.
Separation of Cells (1993) pp. 89-94, Ch. 8, Cell Sorting.
J.R. Lakowicz, “Principles of Fluorescence Spectroscopy”, Plenum Press, New York, pp. 305-309 (1983).
Cardullo et al., “Detection of Nucleic Acid Hybridization by Nonradiative Fluorescence Resonance Energy Transfer”, Proc. Natl. Acad. Sci. USA, vol. 85, pp. 8790-8794, Dec. 1988.
Mergny et al., “Fluorescence Energy Transfer as a Probe for Nucleic Acid Structures and sequenc3es”, Nucleic Acids Research, vol. 22, No. 6, pp. 920-928, 1994.
Sixou et al., “Intracellular Oligonucleotide Hybridization Detected by Fluorescence Resonance Energy Transfer (FRET)”, Nucleic Acids Research, vol. 22, No. 4, pp. 662-668, 1994.
Leonetti et al., “Intracellular Distribution of Microinjected Antisense Oligonucleotides”, Proc. Natl. Acad. Sci. USA, vol. 88, pp. 2702-2706, Apr. 1991.
Fisher et al., “Intracellular Disposition and Metabolism of Fluorescently-Labeled Unmodified and Modified Oligonucleotides Microinjected into Mammalian Cells”, Nucleic Acids Research, vol. 21, No. 16, pp. 3857-3865, 1993.
Sokol et al., “Real Time Detection of DNA-RNA Hybridization in Living Cells”, Proc. Natl. Acad. Sci USA, vol. 95, pp. 11538-11543, Sep. 1998.
Zobel et al., “Cationic Polyhexylcyanoacrylate Nanoparticles as Carriers for Antisense Oligonucleotides”.
J.R. Lakowicz, “Principles of Fluorescence Spectroscopy”, Ch. 10, pp. 303-339, (1983) Plenum Press, New York.
Ishibashi Kaname
Tsuji Akihiko
Hamamatsu Photonics, K.K..
Leydig , Voit & Mayer, Ltd.
Whisenant Ethan
LandOfFree
Method for selectively separating live cells expressing a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for selectively separating live cells expressing a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for selectively separating live cells expressing a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3685997