Drug – bio-affecting and body treating compositions – Plant material or plant extract of undetermined constitution... – Containing or obtained from leguminosae
Reexamination Certificate
2005-08-09
2005-08-09
Witz, Jean C. (Department: 1651)
Drug, bio-affecting and body treating compositions
Plant material or plant extract of undetermined constitution...
Containing or obtained from leguminosae
C424S725000
Reexamination Certificate
active
06926915
ABSTRACT:
The present invention provides a method for selective and high-yield separation, concentration, and recovery of desired cells from a blood sample. The method of the present invention is characterized in that the blood sample is caused to interact with lectins under conditions in which the cell membranes are inactive and cell-lectin complexes
on-complexes are formed, the sample containing these cell-lectin complexes
on-complexes is incubated together with a substrate, the surface of which is covered with polymers having carbohydrate chains which are specifically recognized by the lectins, and the cells are immobilized on the surface of the substrate via the lectins, and subsequently, the liquid layer and the solid phase are separated, and the desired blood cells are recovered from the liquid phase and/or the solid phase, and these lectins are in such an amount that although the cells to be recovered from the solid phase bind to the solid phase with the polymer, the cells to be recovered from the liquid phase do not bind to the solid phase with the polymer.
REFERENCES:
patent: 8-319300 (1996-12-01), None
patent: WO 97/26326 (1997-07-01), None
patent: WO 99/15628 (1999-04-01), None
Tatsuya Sato et al., “An Efficient Method of Bone Marrow Transplantation II. Design of Absorbent Column for Depletion of T Lymphocytes and Concentration of Stem Cells from Donor Bone Marrow”, Journal of Tokyo Jikeikai Ika Daigaku, 1996, vol. 11, No. 1, p. 9-18.
Arnon Nagler, et al., “The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone-Marrow Transplantation”, Molecular Biotechnology, Apr. 1999, vol. 11, No. 2, p. 181-194.
Miercio E. A. Pereira and Elvin A. Kabat, “Immunochemical Studies on Lectins and Their Application to the Fractionation of Blood Group Substances and Cells”, Critical Reviews in Immunology, Nov., 1979, pp. 33-78.
M. Kitagawa, K. Sugiura, T. Okuyama, K. Kanayama, M. Natori, D. Wakamatsu, H. Yura and H. Sago, “A New Method Using Lectin for Isolation of Fetal Cells from Maternal Blood”, American Journal of Human Genetics, Oct. 2000, p. 423.
Michihiro Kitagawa, Kentaro Sugiura, Hiroko Omi, Yoshiaki Akiyama, Kiyoko Kanayama, Masaru Shinya, Tadao Tanaka, Hirobumi Yura and Haruhiko Sago, “New Technique Using Galactose-Specific Lectin for Isolation of Fetal Cells from Maternal Blood”, Prenatal Diagnosis, Jan. 2002, pp. 17-21.
Ihara Akira
Kitagawa Michihiro
Saito Yoshio
Wakamatsu Daisuke
Yura Hirofumi
Netech, Inc.
Pearne & Gordon LLP
Witz Jean C.
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