Method for selectively deactivating catalase while retaining dam

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound having a 1-thia-5-aza-bicyclo

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435184, 435189, 435192, 4352551, C12N 902, C12N 908, C12N 999, C12P 3500

Patent

active

055590063

ABSTRACT:
"A method for selectively deactivating catalase while retaining D-amino acid oxidase activity is disclosed. The catalase and oxidase are both present in whole cells or a cell-free extract. The method comprises combining the whole cells or the cell-free extract with a basic solution at a pH between about 11 and about 12. The catalase activity is eliminated and the oxidase activity is unaffected. This results in the production of a solution which contains oxidase activity but no catalase activity. The whole cells and cell-free extract are preferably from Triginopsis variabilis."

REFERENCES:
patent: 3801458 (1974-04-01), Fildes et al.
patent: 3821209 (1974-06-01), Arnold et al.
patent: 3912589 (1975-10-01), Smith et al.
patent: 3976546 (1976-08-01), Smith et al.
patent: 4079180 (1978-03-01), Suzuki et al.
patent: 4394450 (1983-07-01), Brock et al.
patent: 4745061 (1988-05-01), Aretz et al.
patent: 5424203 (1995-06-01), Vicenzi
Dixon and Webb, Enzymes, 3rd Ed., Academic Press, pp. 11-12 (1979).
Mazzeo et al., J.C.S. Perkin I, p. 2532 (1972).
Agric. Biol. Chem., 45(7) 1561-1567, 1981.
Hakko to Kogyo, vol. 38, No. 3, pp. 216-237 (1980).
Biotechnology Letters, vol. 9, No. 9, 1987, pp. 625-628, S. F. D'Souza et al.
J. of Bioscience, vol. 11, No. 1-4, Mar. 1987, pp. 137-144, A. Deshpande, et al.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for selectively deactivating catalase while retaining dam does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for selectively deactivating catalase while retaining dam, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for selectively deactivating catalase while retaining dam will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1927931

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.