Method for selection of insertion mutants

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 435 911, 4351721, 536 231, 536 2431, 536 2432, C12P 1934, C12Q 168, C07H 2102, C07H 2104

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060134860

ABSTRACT:
The present invention provides a highly-efficient method for the selection and identification of insertional mutants. In the technique, a non-selective amplification is used to isolate a plurality of insertion events from a population of individuals comprising insertion mutations. Specific insertion events can then be identified from the population by the use of gene specific probes or primers. Through the identification of mutants for a particular gene, data may be obtained regarding the function and phenotypic effects of that gene, and thereby, the gene can be employed in the creation of novel biotechnological products.

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