Method for selecting nucleic acids on the basis of structure

Details Method for selecting nucleic acids on the basis of structure Method for selecting nucleic acids on the basis of structure

1994-02-22

1998-01-13

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435962, 435973, 536 254, 935 77, 935 78, C07H 2104, C07H 2102, C12P 1934, C12Q 168

Patent

active

057077967

ABSTRACT:
A method of selecting nucleic acids on the basis of physical structure is disclosed. A modification of the Systematic Evolution of Ligands by Exponential enrichment (SELEX) is used in conjunction with methods for differentiating between nucleic acid molecules on the basis of physical structure, such as chromatography, gel electrophoresis, solubility, and solvent partitioning. The disclosed method may be used in selecting single stranded DNA or RNA, or double stranded DNA or RNA. An example of the method selected nucleic acids of bent DNA. This method represents a new and powerful approach to select nucleic acid molecules with the physical structure required for specific biological activity, for example, in the regulation of gene expression.

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