Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1988-03-11
1995-07-11
Knode, Marian
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352522, 4351721, 4351723, 435 29, 435 34, 435878, 435 37, 935 76, 935 79, C12N 120, C12N 121, C12N 1501
Patent
active
054320798
ABSTRACT:
Methods for the isolation of mutant rhizobial strains with improved competition of nodulation (Comp.sup.+) is presented. Mutants are selected for constitutive expression of inducible nod genes (in the absence of inducer) and screened for hyperinduction of nod genes in the presence of inducer. Mutants which are defective in nodulation or in symbiotic nitrogen fixation are eliminated from further testing. Selections for constitutive nod expression and for hyperinduction of nod genes are facilitated by the use of plasmid carrying a selectable marker fused to an inducible nod gene and a plasmid carrying a reporter gene fused downstream of an inducible nod gene. The methods exemplified are particularly useful for the isolation and identification of Comp.sup.+ mutants of Bradyrhizobium japonicum.
REFERENCES:
patent: 4713330 (1987-12-01), McLoughlin
patent: 4863866 (1989-09-01), Zablotowicz
Burn et al., "Four Classes of Mutations in the nod D gene of Rhizobium Seguminosarum biovar viciae that affect its ability to autoregulate and/or activate other nod genes in the presence of other flavonoid inducers", Genes and Development vol. 1:456-464, 1987.
Nieuwkoop et al. (1987) J. Bacteriol. 169:2631-2638.
Kosslak et al. (1987) Proc. Natl. Acad. Sci. 84:7428-7432.
Spaink et al. (1987) Nature 328:337-340.
Horvath et al. (1987) EMBO J. 6:841-848.
Burn et al. (1987) Genes and Development 1:456-464.
Hong et al. (1987) Nucl. Acids Res. 15:9677-9690.
McLoughlin, U.S. Pat. No. 4,713,330, issued Dec. 15, 1987.
Appelbaum Edward R.
Johansen Eric
Knode Marian
Mycogen Plant Science, Inc.
LandOfFree
Method for selecting mutants of legume-nodulating bacteria enhan does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for selecting mutants of legume-nodulating bacteria enhan, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for selecting mutants of legume-nodulating bacteria enhan will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-502876