Method for selecting monoclonal antibodies for human G-CSF permi

Details Method for selecting monoclonal antibodies for human G-CSF permi Method for selecting monoclonal antibodies for human G-CSF permi

1995-06-07

1998-08-25

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

435 792, 435 793, 435 794, 435 795, 435 7021, 436548, 53038823, G01N 33531, G01N 33543

Patent

active

057982740

ABSTRACT:
The present invention relates to a high affinity monoclonal antibody for human G-CSF and to an enzyme immunoassay using said antibody. Specifically, the practice of the invention permits one skilled in the art to obtain monoclonal antibodies with the characteristics necessary to permit low-level detection of G-CSF in plasma of equal to or less than 0.5 pg/ml in an immunoassay without interference in human fluids.

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Watari, K. et al., "Serum Granulocyte Colony-Stimulating Factor Levels in Healthy Volunteers and Patients with Various Disorders as Estimated by Enzyme Immunoassay," Blood, 73:117-122 (1989).

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