Method for selecting genetically transformed cells

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S468000

Reexamination Certificate

active

07449290

ABSTRACT:
An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-transformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non-transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

REFERENCES:
patent: WO A 93 05163 (1993-03-01), None
patent: WO A 94 20627 (1994-09-01), None
patent: WO 96 31612 (1996-10-01), None
Roger A. Aeschbacher et al.,Purification of the Trehalase GMTREI From Soybean Nodules and Cloning of Its cDNA GMTREI Is Expressed at a Low Level in Multiple Tissue,Plant Physiology, Feb. 1999, vol. 119, pp. 489-495.
Joachim Muller et al.,Trehalose and Trehalase in Arabidopsis,Plant Physiology, Feb. 2001, vol. 125, pp. 1086-1093.
Naoki Asano et al.,Effect of Validamycins on Glychohydrolases of Rhizoctonia Solani,The Journal of Antibiotices, Apr. 1987, pp. 526-532.
Irene Knuesel et al.,Comparative Studies of Suidatrestin, A Specific Inhibitor of Trehalases,Comparative Biochemistry and Physiology Part B 120, 1998, pp. 639-646.
Reinhold et al., Characterization of a Cytoplasmic Trehalase ofEscherichia coli,Journal of Bacteriology, Nov. 1996, p. 6250-6257.

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