Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 25 or more amino acid residues in defined sequence
Patent
1999-07-07
2000-12-12
Guzo, David
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
25 or more amino acid residues in defined sequence
530326, 530327, C07K 14195
Patent
active
06160089&
ABSTRACT:
A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.
REFERENCES:
Kashiwagi K. et al.: "Identification of the polyamine-induced protein as a periplasmic oligopeptide binding protein" J. Biol. Chem., vol. 265, No. 15, May 25, 1990, pp. 8387-8391.
Van Dijl et al.: "Signal peptidase I of Bacillus subtilis: patterns of conserved amino acids in prokaryotic and eukaryotic type I signal peptidases" EMBO Journal, GB, Oxford University Press, Surrey, vol. 11, No. 8, Jan. 1, 1992, pp. 2819-2828.
Zhang Y-B et al.: "Analysis of a Streptococcus pneumoniae gene encoding signal peptidase I and overproduction of the enzyme" GENE: An International Journal On Genes And Genomes, vol. 194, No. 2, Jul. 31, 1997, pp. 249-255.
Honjo Masaru
Matsumoto Kazuya
Mochizuki Daisuke
Naitoh Naokazu
Uchida Hiroshi
Guzo David
Leffers, Jr. Gerald G.
Mitsui Chemicals Inc.
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