Method for screening substance inhibiting activation of NF-.kapp

Details Method for screening substance inhibiting activation of NF-.kapp Method for screening substance inhibiting activation of NF-.kapp

1999-03-19

2000-12-05

McKelvey, Terry

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435243, 4353201, 435325, 435410, 536 241, C12Q 168, C07H 2104, C12N 100, C12N 510, C12N 1563

Patent

active

061565164

ABSTRACT:
An expression regulatory sequence comprising a NF-.kappa.B recognition sequence, at least part of the 3'-untranslated region (3'-UTR) and at least part of the 3'-flanking region of human induced nitrogenmonoxide synthase (hiNOS) gene; and a method for screening a substance repressing the activation of NF-.kappa.B, comprising treating cells having the expression regulatory sequence and capable of detecting the activation of NF-.kappa.B with a subject substance, and observing a change in the expression amount of reporter genes.

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