Method for screening restriction endonucleases

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S199000

Reexamination Certificate

active

06905837

ABSTRACT:
A method is provided for identifying a restriction endonuclease, which includes the steps of (a) screening a target DNA sequence for the presence of known methylase sequence motifs, (b) identifying any open reading frames which lie close to the methylase sequence motifs screened in step (a), and (c) assaying the protein products of these open reading frames for restriction endonuclease activity.Methods for identifying isoschizomers of known restriction endonucleases, which isoschizomers possess a desired physical property, such as thermostability, are also provided by the present invention, as are several novel restriction endonucleases isolated fromM. jannaschii, MjaIII and MjaIV. Additionally, a gene was identified that encoded a previously observed endonuclease activity, designated MjaII.Also provided by the present invention are vectors suitable for cloning a DNA sequence encoding a cytotoxic protein, via independent transcription promotors which may be selectively controlled by several conditions. A method for producing these cytotoxic proteins using such vectors is also provided, as are stable clones of PacI and NlaIII.

REFERENCES:
patent: 5098839 (1992-03-01), Polisson
patent: 5179015 (1993-01-01), Wilson et al.
patent: 5180673 (1993-01-01), Wilson et al.
patent: 5278060 (1994-01-01), Morgan
patent: 0 456 356 (1994-04-01), None
O'Connor and Timmis, Journal of Bacteriology, 169:4457-4462 (1987).
Bickle, Nucleases [eds. Linn, S.M., Lloyd, S.L., and Roberts, R.J.], Cold Spring Harbor Laboratory Press, pp. 89-109 (1993).
Smith and Wilcox, J. Mol. Biol. 51:379-391 (1970).
Kelly and Smith, J. Mol. Biol. 51:393-409 (1970).
Middleton, et al., J. Viori. 10:42-50 (1972).
Roberts, et al., J. Mol. Biol., 91:121-123 (1975).
Schildkraut, “Screening For And Characterizing Restriction Endonucleases” in Genetic Engineering, Principles and Methods, vol. 6, pp. 117-140 Plenum Press (1984).
Roberts and Macelis, Nucl. Acids Res. 26:338-350 (1998).
Szomolanyi, et al., Gene 10:219-225 (1980).
Mann, et al., Gene 3:97-112 (1978).
Kiss, et al., Nucl. Acids Res. 13:6406-6420 (1985).
Brooks, et al., Nucl. Acids Res. 17:979-997 (1989).
Elledge and Davies, Genes & Develop. 3:185-197 (1988).
Elledge, et al., PNAS USA 86:3689-3693 :(1989).
Dorner and Schildkraut, Nucl. Acids Res. 22:1068-1074 (1994).
Wilson, et al., Nucl. Acids Res. 19:2539-2566 (1991).
Lubys, et al., Gene 141:85-89 (1994).
Withers, et al., Nucl. Acids Res. 20:6267-6273 (1992).
Posfai, et al., Nucl. Acids Res. 17:2421-2435 (1989).
Lauster, et al., J. Mol. Biol. 206:305-312 (1989).
Wilson, Meth. Enzymol., 216:259-279 (1992).
Timinskas, et al., Gene 157:3-11 (1995).
Fleischmann, et al., Science 269:496-512 :(1995).
Fraser, et al., Science 270:397-403 (1995).
Bult, et al., Science 273:1058-1073 (1996).
Himmelreich, et al., Nucl. Acids Res. 24:4420-4449 (1996).
Kaneko, et al., DNA Res. 3:109-136 (1996).
Altschul, et al., J. Mol. Biol., 215:403-410 (1990).
Pearson and Lipman, PNAS USA 85:2444-2448 (1988).
Bolivar, et al., Gene 2:95-113 (1977).
Horiuchi and Inokuchi, Journal of Molecular Biology 23:217-224 (1967).
Nolling etal., Nucleic Acids Res. 20:5047-5052 (1992).
Nolling, et al., Nucleic Acids Res. 20:6501-6507 (1992).
Roberts and Halford in Nucleases [eds. Linn, S.M., Lloyd, R.S., Roberts, R.J] Cold Spring Harbor Press, pp. 35-88 (1993).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for screening restriction endonucleases does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for screening restriction endonucleases, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening restriction endonucleases will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3515130

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.