Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Reexamination Certificate
2003-09-04
2010-06-15
Joike, Michele K (Department: 1636)
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
C435S198000, C435S320100, C435S254110, C530S350000, C536S023100
Reexamination Certificate
active
07736643
ABSTRACT:
The present invention relates to a method for screening of the lipase having improved enzymatic activity using yeast surface display vector and the mutant lipase prepared by the same, more particularly to the method comprising; 1) cloning lipase gene into surface display vector, 2) preparing mutant lipase gene library of the step 1 by mutagenic PCR, 3) transforming the mutant lipase gene library of the step 2 and surface display vector into host cell, and 4) measuring the activity of the mutant lipase displayed in the surface of the transformed host cell and selecting the mutant lipase prepared by the same. The method of the present invention can screen the lipase having improved enzymatic activity. So, it can effectively be used for the various fields, such as food and detergent industry.
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Choi Eui-Sung
Kim So-Young
Sohn Jung-Hoon
Joike Michele K
Korea Research Institute of Bioscience and Biotechnology
The Nath Law Group
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