Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-10-13
1998-10-20
Schwadron, Ronald B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 721, 435 724, 435 74, 530300, 530327, 530350, 530827, 5303881, 5303891, 530395, 530402, 530412, 530413, 436501, 436503, 436518, 436536, G01N 3353, G01N 33567, G01N 33536, C07K 708
Patent
active
058244875
ABSTRACT:
This invention provides a method for identifying a cellular protein capable of specifically binding to an activated antibody receptor, whose cytoplasmic domain comprising an ARH1 motif, comprising (a) obtaining cells comprising receptors having the ARH1 motif; (b) lysing the cells under conditions whereby the native complex of the receptor having the ARH1 motif and the cellular protein is preserved;(c) isolating the complex; and (d) testing the associated receptor and the protein for biochemical activities, thereby identifying the cellular protein capable of specifically binding to an activated antibody receptor, whose cytoplasmic domain comprising an ARH1 motif. This invention further provides a method for identifying a cellular molecule capable of being a target for designing drugs for autoimmune disease, inflammation or allergy which comprises (a) contacting a cell lysate with a molecule having a motif of amino acid sequence, AENTITYSLLKHP under the conditions permitting formation of a complex between the cellular target molecule with the motif; (b) isolating the complex formed in step (a); and (c) testing the complex for biochemical activities, thereby identifying the cellular molecule capable of being a target for designing drugs for autoimmune disease, inflammation or allergy.
REFERENCES:
Clark, M., et al. (1992) The B Cell Antigen Receptor Complex: Association of Ig-.alpha. and Ig-.beta. with Distinct Cytoplasmic Effectors. Science, 258: 123-126.
Eiseman, E. et al. (1992) Signal Transduction by the Cytoplasmic Domains of FC.epsilon.RI-.gamma. and TCR-.zeta. in Rat Basophilic Leukemia Cells. The Journal of Biological Chemistry, 267: 21027-21032.
Irving, B. et al. (1991) The Cytoplasmic Domain of the T Cell Receptor .zeta. Chain Is Sufficient to Couple to Receptor-Associated signal Transduction Pathways. Cell, 64:891-901.
Karnitz, L., et al. (1992) Effects of p. 56.sup.lcK Deficiency on the Growth and Cytolytic Effector Function of an Interleukin-2-Dependent Cytotoxic T-Cell Line. Molecular and Cellular Biology, 12:4521-4530.
Letourneur, F. et al. (1991) T-cell and basophil activation through the cytoplasmic tail of T-cell receptor .zeta. family proteins. Proc Natl. Acad. Sci, USA, 88:8905-8909.
Orloff, G. et al. (1990) Family of disulphide-linked dimers containing the .zeta. and .eta. chains of the T-cell receptor and the .gamma. chain of Fc receptors. Nature, 347: 189-191.
Romeo, C. et al. (1991) Cellular Immunity to HIV Activated by CD4 Fused to T Cell or Fc Receptor Polypeptides. Cell, 64:1037-1046.
Straus, D. et al. (1992) Genetic Evidence for the Involvement of the Ick Tyrosine Kinase in Signal Transduction through the T Cell Antigen Receptor Cell, 70:585-593.
Edsington, Bio/Technology, 10: 383-389, 1992.
Kurosaki Tomohiro
Ravetch Jeffrey V.
Schwadron Ronald B.
Sloan-Kettering Institute for Cancer Research
White John P.
LandOfFree
Method for screening for targets for anti-inflammatory or anti-a does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for screening for targets for anti-inflammatory or anti-a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening for targets for anti-inflammatory or anti-a will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-242958