Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2007-06-05
2007-06-05
Andres, Janet L. (Department: 1649)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007200, C435S007210, C435S007800, C435S007900, C435S007930, C436S501000, C436S503000
Reexamination Certificate
active
09806842
ABSTRACT:
In methods for screening treatments for, and treatment of, neurodegenerative diseases, aggregation in neurons of NACP/α-synuclein is measured and expression of a non-amyloidogenic protein is stimulated in order to reduce the level aggregration. For purposes of screening agents for treatment of neurodegenerative disease, oxidative stress in the neuronal cells is stimulated by introducing a mixture of metal-ions and hydrogen peroxide. Examples of appropriate metals include iron, aluminum, and copper. After introduction of the agent under evaluation for stimulation of expression of non-amyloidogenic protein, the effectiveness is measured by testing for a decrease in the level of aggregation of NACP/α-synuclein. In an exemplary embodiment, the non-amyloidogenic protein is β-synuclein. The aggregation of NACP/α-synuclein is dependent upon the concentration of metal ions in the neuronal cells. In addition, the presence of chelating agents appears to modulate the build-up of NACP/α-synuclein aggregates which are responsible for synaptic and neuronal dysfunction.
REFERENCES:
patent: 6184351 (2001-02-01), Biere et al.
patent: 6504080 (2003-01-01), Van Der Putten
patent: 6780971 (2004-08-01), Wolozin et al.
Hashimoto et al., NeuroReport 10:717-721, 1999.
Jensen et al., Biochem. J., 323:539-546, Apr. 15, 1997.
Harris et al., Experimental Neurology, 1995, 131(2):193-202.
Hashimoto et. al., The Journal of Biological Chemistry 274(41):28849-28852, 1999.
Narhi et. al., The Journal of Biological Chemistry, 274(14):9843-9846, 1999.
RG Perez and TG Hastings, Journal of Neurochemistry, 89(6):1318, 2004.
Jenner et al (Sep. 1998;). Annals of Neurology. 44(suppl. 1), S72-S84.
Lutz (1990). Mut. Res., 238, (abstract only).
Korge et al (1993). Archives of Biochemistry and Biophysics. 304, 420-428.
Bauminger et al (1999) Biochemistry. 38, 7791-7802.
Guptasarma et al (1992). Biochemistry. 31, 4296-4303.
Spillantini, M.G. et al. α-Synuclein in Lewy bodies. Nature. 388:839-40. 1997.
Hashimoto Makoto
Masliah Eliezer
Rockenstein Edward
Andres Janet L.
Morrison & Foerster / LLP
Standley Steven H.
The Regents of the University of California
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