Combinatorial chemistry technology: method – library – apparatus – Method of screening a library – By measuring the effect on a living organism – tissue – or cell
Reexamination Certificate
2006-04-17
2009-12-01
Low, Christopher (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of screening a library
By measuring the effect on a living organism, tissue, or cell
C506S018000, C506S007000, C435S007200
Reexamination Certificate
active
07625844
ABSTRACT:
The instant invention provides a live labeling microorganism assay for screening a one-bead one-compound library to identify synthetic anti-adherent compounds for blocking biofilm formation. The compounds of libraries may be peptides, small molecules, nucleic acids, other types of molecules or a combination of the foregoing. The live labeling microorganisms are incubated with a one-bead one-compound library for a long period with fresh-labeled bacteria and nutrition replacement in regular intervals. Those beads without bacterial adhesion are transferred into nutritional agar for the culture. The true anti-adherent beads are isolated and the sequences of each isolated compound-bead are determined with sequencer. The anti-adherent compounds identified in this invention are proved to possess long-term effect of blocking bacterial adhesion and biofilm formation. Thus the instant invention provides a unique and powerful method to identify anti-adherent compounds from library more quickly and reliably than current state-of-the-art technology allows.
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Yang Fan
Yang Guang
Andrews & Kurth LLP
Biopath Laboratory Incorporated (Delaware)
Chao Fei-Fei
Gross Christopher M.
Low Christopher
LandOfFree
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