Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2006-07-25
2006-07-25
Witz, Jean C. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C435S007700, C435S195000, C435S184000, C435S200000
Reexamination Certificate
active
07081351
ABSTRACT:
The present invention provides a method for testing an agent for its potential to inhibit heparanase catalytic activity, comprising the steps of: interacting immobilized heparanase substrate binding protein on a solid support with labeled heparanase substrate to provide immobilized labeled heparanase substrate, interacting a heparanase enzyme solution with the immobilized labeled heparanase substrate in the presence of the agent, and detecting the presence or absence of label in the solution remote from the solid support. The labeled heparanase substrate may be bound to an immobilization bridge which binds to immobilized molecules on a solid support, to provide bridge-immobilized labeled heparanase substrate. The present invention also provides a method for testing an agent for its ability to inhibit binding between fibroblast growth factor (FGF) and heparanase substrate, comprising the steps of: interacting in solution immobilized FGF on a solid support with the agent and labeled heparanase substrate, and detecting the presence or absence of label in the solution remote from the solid support.
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Huang Kuo-Sen
Neri Anthony
Roberts John L
Hanley Susan
Hoffmann-La Roche Inc.
Johnson George W.
Rocha-Tramaloni Patricia S.
Wildman David E.
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