Method for sampling and measuring the content of a low-molecular

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid

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435 12, 435 14, 435 18, 435 28, 23230B, C12Q 162, C12Q 158, C12Q 154

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043117899

ABSTRACT:
A method is provided for sampling and measuring the content of a low-molecular weight compound, such as glucose, cholesterol, or urea, in a complex fluid medium to be sampled, such as blood. A dialyzer is placed in contact with the complex fluid medium so that it passes through the dialyzer. One or more semipermeable membranes are disposed within the complex fluid medium to form one or more passageways for the passage of dialysis fluid, with the passageways being substantially smaller in volume than the volume of the surrounding complex fluid medium. A fractional portion of the complex fluid medium is acted on by the dialysis fluid and dialyzed so that only a fractional portion of the low-molecular weight compound diffuses into the passageways containing the dialysis fluid to produce a dialysate in the passageways. The dialysate is then measured to determine the content of the low-molecular weight compound in the complex fluid medium.

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