Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-02-25
1996-01-09
Bension, Gary
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 43524046, 43524049, 800200, 800DIG47, 800DIG50, A01H 400, A01H 700, C12N 501
Patent
active
054828570
ABSTRACT:
The invention is a method for reproducing Douglas-fir by somatic embryogenesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage embryos. These are multiplied in a second culture having reduced growth hormones where the early stage embryos grow in size and vigor to advanced early stage embryos. The embryos may then be transferred directly to a cotyledonary embryo development culture containing an adsorbent such as activated charcoal. Preferably, they are first singulated by shaking in two or more liquid subcultures containing the adsorbent prior to placing them on the development medium. After several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth.
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Gupta Pramod K.
Pullman Gerald S.
Bension Gary
Weyerhaeuser Company
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