Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1990-03-26
1991-07-30
Locker, Howard J.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 43524046, 43524047, 43524049, 43524054, 4351721, 800200, 800DIG49, 800DIG50, 800DIG51, A01H 400, A01H 700, C12N 504
Patent
active
050360078
ABSTRACT:
The invention is a method for developing tissue culture induced coniferous somatic proembryos into well developed cotyledonary embryos. The method comprises a multistage culturing process in which early stage proembryos are cultured on a late stage proembryo medium comprising a significantly higher osmotic potential along with abscisic acid and an absorbent material to gradually reduce the level of available abscisic acid over time. Culturing from this point continues in an embryo development medium having a high osmotic potential in which the osmotic potential is preferably raised during embryo development to a final level of about 450 mM/kg. Through this process the vigor and morphology of the embryos is improved and the tendency to germinate prematurely is significantly reduced. After a period of several weeks in culture somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to the soil for further growth.
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Gupta Pramod K.
Pullman Gerald S.
Benzion Gary
Locker Howard J.
Weyerhaeuser Company
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