Method for rendering streptokinase less immunogenic to a human

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S183000, C424S094100, C424S133100

Reexamination Certificate

active

07125689

ABSTRACT:
Protein, or parts of proteins, may be rendered non-immunogenic, or less immunogenic, to a given species by identifying in their amino acid sequences one or more potential epitopes for T-cells of the given species and modifying the amino acid sequence to eliminate at least one of the T-cell epitopes. This eliminates or reduces the immunogenicity of the protein when exposed to the immune system of the given species. Monoclonal antibodies and other immunoglobulin-like molecules can particularly benefit from being de-immunised in this way: for example, mouse-derived immunoglobulins can be de-immunised for human therapeutic use.

REFERENCES:
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Bruserud et al. Journal of Clinical and Laboratory Immunology 20(2):69-74, 1986.
Eyerman et al. Journal of immunology 152:1569-1577, 1994.

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