Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-06-20
1996-07-02
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 435 911, 435810, 536 221, 536 231, 536 253, 935 77, 935 78, 935 88, C12P 1934, C07H 2100
Patent
active
055321461
ABSTRACT:
Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
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Cimino George D.
Isaacs Stephen T.
Tessman John W.
HRI Research Inc.
Jones W. Gary
Marschel Ardin H.
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