Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1989-05-02
1992-08-04
Russel, Jeffrey E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530350, 5303871, 530404, 530408, 5303911, 5303905, C07K 312
Patent
active
051360276
ABSTRACT:
A high concentration of renaturing surfactants is added to protein systems in the presence of alkyl sulfate detergents to displace the detergent with respect to the interaction with the protein, thereby renaturing the protein and restoring its reactivity. The presence of higher quantities of detergent in the system results in smaller protein aggregates, and thus a higher reactivity and specificity as measured for the system in its entirety.
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patent: 4797474 (1989-01-01), Patroni et al.
patent: 4801691 (1989-01-01), Auer
patent: 4966963 (1990-10-01), Patroni
Kuusi et al, "Immunochemical Characterization of Major Outer Membrane Components from Salmonella typhimurium," Infection and Immunity, vol. 33, No. 3, Sep. 1981, pp. 750-757.
Ernst J. Am. Oil Chem. Soc. 57:93 (1980).
Furth, Analytical Biochemistry, 109:207-215 (1980).
Neugepauer, A Guide to the Properties and Uses of Detergents in Biology and Biochemistry (1987).
Abbott Laboratories
Brainard Thomas D.
Russel Jeffrey E.
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