Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1987-05-15
1989-01-31
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
435212, 435226, 530305, 530344, 530351, 530380, 530412, 530408, 530416, 530417, 530422, C07K 324, C07K 328
Patent
active
048016918
ABSTRACT:
Guanidine hydrochloride (GCl) is used to remove excess sodium dodecyl sulfate (SDS) from SDS-solubilized protein solutions, and particularly from SDS-solubilized inclusion body solutions, GCl is added to the solution containing SDS to induce the formation of a GCl-SDS complex (GDS) which, when allowed to precipitate, can easily be removed by centrifugation, filtration, or other suitable means.
REFERENCES:
patent: 4058510 (1977-11-01), Concilio-Nolan et al.
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4518526 (1985-05-01), Olson
patent: 4530787 (1985-07-01), Shaked et al.
patent: 4569790 (1986-02-01), Koths et al.
patent: 4572798 (1986-02-01), Koths et al.
patent: 4620948 (1986-11-01), Builder et al.
patent: 4677196 (1987-06-01), Rausch et al.
patent: 4766224 (1988-08-01), Rausch
Kapp et al., Removal of Sodium Dodecyl Sulfate from Proteins, Analytical Biochemistry 91, 230-235 (1978).
Putnam, The Interactions of Proteins and Synthetic Detergents, Advances in Protein Chemistry, vol. 4, 79-122(1948).
Farquer Thomas L.
Guffey Wendell R.
International Minerals & Chemical Corp.
Schain Howard E.
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