Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1988-05-16
1992-03-31
Schwartz, Richard A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
424 92, 435 713, C07K 324, C07K 328, A61K 3910, C12P 2102
Patent
active
051010194
ABSTRACT:
The present invention provides a clear and efficient pertussis endotoxin-removing method characterized in that a fluid containing the antiinfective fraction and endotoxin of strains of phase I Bordetella pertussis is supplied with calcium ion in the presence of excess phosphate ion prior to zonal centrifugation and also a pertussis toxoid whose endotoxin content per 10 .mu.g of proteinic nitrogen is not more than 0.5 ng and its production method.
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Work, E. 1971, "Production, Chemistry, and Properties of Bacterial Pyrogens and Endotoxins", CIBA Foundation Symposium Pyrogens and Fever, eds. Wolstenholme, G. E. W. et al., Churchill Livingston, Edinburgh, Scotland, pp. 23-47.
Knox, K. W. et al., 1967, "An Extracellular Lipopolysaccharide-Phospholipid-Protein Complex . . . ", Biochem. J., vol. 103, pp. 192-201.
Watanabe, H. et al., 1983, "An Improvement in the Method of Purification of B. Pertussis LPF-HA Ca Pertussis Toxin and F-HA and a Method (PHA) for Assay of Their Antibodies", Japanese Journal of Bacteriology, vol. 38 (1) p. 423.
Hideo Watanabe et al., Japanese Journal of Bacteriology, 38 (1), 423 (1983).
Fujita Isao
Miyamoto Masatoshi
Watanabe Hideo
Mosher Mary E.
Schwartz Richard A.
Takeda Chemical Industries Ltd.
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