Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1995-05-19
1996-10-15
Rollins, John W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435212, 435223, C12P 2106, C12N 948, C12N 958
Patent
active
055653308
ABSTRACT:
A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with a dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium descoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3.5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.
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Atkinson Paul R.
Hilton Matthew D.
Lambooy Peter K.
Eli Lilly and Company
Maciak Ronald S.
Norman Douglas K.
Rollins John W.
Weber Jon P.
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