Method for removing N-terminal dipeptides from precursor polypep

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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435212, 435223, C12P 2106, C12N 948, C12N 958

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active

055739237

ABSTRACT:
A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with an immobilized dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium discoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3.5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.

REFERENCES:
patent: 5126249 (1992-06-01), Becker et al.
Chan, et. al., 1985, Biochem. Biophys. Res. Comm., 127(3) : 962-968.
Chan, et. al., 1987, Experimental Mycology, 11:27-35.
Huang, et. al., 1992, Experimental Mycology, 16:102-109.
Hutchinson et al, (1987) Biochim. Biophys. Acta., 916, 1-4.
Erickson et al, (1983) Biochim. Biophys. Acta., 743, 37-42.

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