Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1993-12-22
1996-11-12
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435212, 435223, C12P 2106, C12N 948, C12N 958
Patent
active
055739237
ABSTRACT:
A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient time to remove the dipeptide with an immobilized dipeptidylaminopeptidase (dDAP) from the slime mold, Dictyostelium discoideum, which has a mass of about 225 kilodaltons and a pH optimum of about 3.5. The precursor polypeptides may be made recombinantly and may be analogs of naturally occurring polypeptides.
REFERENCES:
patent: 5126249 (1992-06-01), Becker et al.
Chan, et. al., 1985, Biochem. Biophys. Res. Comm., 127(3) : 962-968.
Chan, et. al., 1987, Experimental Mycology, 11:27-35.
Huang, et. al., 1992, Experimental Mycology, 16:102-109.
Hutchinson et al, (1987) Biochim. Biophys. Acta., 916, 1-4.
Erickson et al, (1983) Biochim. Biophys. Acta., 743, 37-42.
Atkinson Paul R.
Foster Lisa K.
Boone David E.
Eli Lilly and Company
Maciak Ronald S.
Weber Jon P.
Wityshyn Michael G.
LandOfFree
Method for removing N-terminal dipeptides from precursor polypep does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for removing N-terminal dipeptides from precursor polypep, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for removing N-terminal dipeptides from precursor polypep will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-561735