Method for removing antifoaming agents during processing of micr

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of...

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435183, 435246, 435812, 435814, 210691, 252321, C12N 100, C12N 900, C12N 134

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active

049313975

ABSTRACT:
Antifoaming agents are commonly used during culturing of enzyme-producing microorganisms. These antifoams often persist through enzyme processing, slowing filtrations, clogging filtration membranes and adversely affecting the quality of the final product. The present invention describes a method for removing antifoams, and often carbohydrates and pigments, from enzyme systems using mineral clay, the preferred clay being bentonite.

REFERENCES:
patent: 1939937 (1933-12-01), Werner
patent: 2291624 (1942-08-01), Heimann et al.
patent: 3408306 (1968-10-01), Boylan
patent: 4548803 (1985-10-01), Dickey
patent: 4657859 (1987-04-01), Kelemen et al.
Zaborsky, Immobilized Enzymes, CRC Press, Cleveland, Ohio, 1973, pp. 75-81.
Peppler (editor), Microbial Technology, Reinhold Publishing, New York, 1967, pp. 375-377.
Hedman et al., "Enzyme Recovery by Adsorption From Unclarified Microbial Cell Homogenates", In: Enzyme Engineering 7, Laskin et al. (editor).
Annals of the New York Academy of Sciences, vol. 434, Dec. 31, 1984, pp. 285-288.

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