Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1997-04-08
1999-01-05
Naff, David M.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435420, 435430, 4354301, 435431, C12N 500
Patent
active
058561919
ABSTRACT:
The invention relates to a method for regeneration of plants of the genus Pinus by culturing explants of immature zygotic embryos on culture initiation medium containing abscisic acid, nutrients, growth hormones, sugar and a gelling agent to grow embryogenic tissue for cryopreservation. Culturing of the embryogenic tissue is continued on culture maintenance medium, embryo development medium, and germination medium. The germinated embryos are further converted to acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.
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Kerr Janet M.
McDaniel Terry B.
Naff David M.
Reece IV Daniel B.
Schmalz Richard L.
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