Method for regeneration of adsorbent matrices

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

Reexamination Certificate

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C210S656000, C210S198200, C502S403000

Reexamination Certificate

active

07052609

ABSTRACT:
The present invention relates to the regeneration of adsorbent matrices that are used for the purification of substances from aqueous liquid samples derived from biological material. The method is adapted to adsorbent matrices that are sensitive to hydrolysis either because of a sensitive base matrix or a sensitive ligand including also a sensitive spacer and is characterized in contacting at least one regeneration solution with the adsorbent matrix which comprises an organic solvent which is water-miscible and has a pH value ≦4, preferably ≦3 (acid solution) or ≧10 but <13 (alkaline solution).

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patent: WO 01/27623 (2001-04-01), None
“Streamline rProtein A” Amersham Biosciences, 2002, pp. 1-6, XP002233246.
Kenny Sproule, et al. “New Strategy for the Design of Ligands for the Purification of Pharmaceutical Proteins by Affinity Chromatography”, Journal of Chromatography B, vol. 740, 2000 pp. 17-33.
Jennifer M. Sokol, et al. “Aprotinin conformational distributions during reversed-phase liquid chromatography analysis by hydrogen-exchange mass spectrometry”, Journal of Chromatography A, vol. 1007, 2003, pp. 55-66.

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