Method for regenerating glucose oxidase reagent

Details Method for regenerating glucose oxidase reagent Method for regenerating glucose oxidase reagent

1975-03-20

1977-04-19

Tanenholtz, Alvin E.

Textiles: fiber preparation

G01N 3114

Patent

active

040186512

ABSTRACT:
A glucose oxidase reagent used in an instrumental analyzer, wherein glucose concentration is determined as directly proportional to the rate of oxygen comsumption by glucose in a sample of a biological fluid, is regenerated by mixing and collecting the used reagent in a waste bottle containing sufficient catalase to provide a concentration of at least 2 U/ml of catalase in the total amount of waste liquid. A new glucose oxidase reagent formulation is also provided which contains at least 90 U/ml of catalase and can be continuously recycled by passing the used reagent directly back into the fresh reagent without first collecting in a waste bottle.

REFERENCES:
patent: 3677903 (1972-07-01), Bittner
patent: 3838011 (1974-09-01), Hagen et al.
patent: 3857771 (1974-12-01), Steinberg
Aebi, "Catalase," Method of Enzymatic Analysis by H. H. Bergmeyer, Academic Press, Inc., 2nd Ed., (1974), vol. 2, pp. 673-674.

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