Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-12-13
1998-02-03
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 530427, C12N 950, C12P 2106, C07K 100
Patent
active
057143714
ABSTRACT:
A method for solubulizing and refolding insoluble aggregates of HCV protease is presented. Insoluble aggregates of HCV NS3 protease are extracted from bacteria producing the aggregates. The aggregates of HCV NS3 are then solubilized in a buffer containing the denaturing reagent. Solubilized protease is then placed in an acidic buffer containing a reducing agent. The denaturing reagent is then removed from the buffer under acidic conditions. The pH of the buffer containing HCV NS3 protease is then raised in a step-wise manner to a pH of about 7-8 so as to produce properly refolded soluble, active HCV NS3 protease.
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patent: 4931543 (1990-06-01), Halenbeck et al.
patent: 4977248 (1990-12-01), Creighton et al.
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N.B. Cited references were provided with copending application 08/439,680.
Ramanathan Lata
Wendel Michele
Dulak Norman C.
McLaughlin Jaye P.
Schering Corporation
Weber Jon P.
Wityshyn Michael G.
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