Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2007-02-07
2010-06-08
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S226000
Reexamination Certificate
active
07732183
ABSTRACT:
The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1° C. to 15° C., and incubating the diluted sample at a temperature of about 4° C. to 15° C. until the recombinant BACE polypeptide folds into an active enzyme.
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Emmons Thomas
Heinrikson Robert
Mildner Ana
Paddock Donna
Tomasselli Alfredo
Elan Pharmaceuticals Inc.
McDonnell Boehnen & Hulbert & Berghoff LLP
Nashed Nashaat T
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