Method for reduction of transgene copy number

Details Method for reduction of transgene copy number Method for reduction of transgene copy number

1997-12-22

2000-11-28

Smith, Lynette R. F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

435 6, 435 691, 435468, 435470, 800290, 800295, 800292, 800293, 800294, 800305, 800306, 800312, 800316, 8003172, 8003173, 800320, 8003201, 8003202, 8003203, 800322, 800314, C12N 1582, C12N 1584, A01H 400, A01H 500

Patent

active

061538116

ABSTRACT:
The present invention provides methods for the efficient production of transformants with low transgene copy numbers. In the method, the average transgene copy number of transformants is decreased through methods which are believed to limit the interaction between segments of transforming DNA prior to transformation. The methods comprise means for end-modification of transforming DNA and use of limited quantities of DNA for transforrnation. Production of single or low copy transformation events is desirable in that it avoids many of the problems associated with high transgene copy number including co-suppression, unpredictable gene expression and non-Mendelian inheritance.

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