Method for reducing inhibitors of nucleic acid hybridization

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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Details

435 6, 435259, 4351739, 514514, C12Q 168, C12N 106, C12N 1300, A01N 3712

Patent

active

057631859

ABSTRACT:
The present invention relates to a method for reducing the amount of substances inhibitory to nucleic acid hybridization in samples. The method is practiced prior to release of target nucleic acid from cells of interest and involves contacting the sample with an agent which solubilizes the inhibitory substances and does not effectuate release of nucleic acids from cells in the sample, and then the cells from the agent.

REFERENCES:
patent: 5482834 (1996-01-01), Gillespie et al.
patent: 5554503 (1996-09-01), Down et al.
patent: 5571894 (1996-11-01), Weis et al.

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