Method for reducing effect of hematocrit on measurement of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S014000, C435S013000, C435S269000

Reexamination Certificate

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07323315

ABSTRACT:
A method for determining the presence and/or amount of an analyte in a sample of whole blood comprises the step of treating the sample with a nonlytic hypertonic salt composition to reduce the hematocrit by reducing the size of the red blood cells. In optical detection systems, the smaller red blood cells create greater scatter, which allows a more accurate correction to be applied in a dual-wavelength detection system. In electrochemical detection systems, as well as in optical detection systems, the smaller red blood cells provide less obstruction to the diffusion of analyte and reagents in the sample, to facilitate the reactions thereof.

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Red Cell Volume in Glycerol/Sodium Chloride/Water Mixtures, Brief Communications, Cryobiology 21, pp. 234-239 (1984).
European Search Report, Nov. 8, 2005.

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