Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-01-23
1999-03-02
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 935 77, 935 78, C12P 1934, C12Q 168
Patent
active
058769765
ABSTRACT:
The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4876187 (1989-10-01), Duck et al.
patent: 5334515 (1994-08-01), Rashtchian et al.
Longo et al., Gen. 93(1990) 125-128.
Syvanen et al., Nucl. Acids Res. 16(23), 11327-11338 (1988).
Vosberg, Hum. Gen., 83(1), 1-1-15 (1989).
Orlandi et al., Proc. Natl. Acad. Sci. USA 86 (1989), 3833-3837.
Wong et al., Nat. (1987), 330-384.
Kwok et al., J. Virol., (1987), 61-1690.
Bos et al, Nat., (1987), 327-293.
Mullis, K. and Faloona, f., Methods in Enzymology, (1987) 155-335.
Ou et al, Sci., (1988), 239-295.
Saiki et al, Sci., (1988), 230-1350.
Engelke et al, Proc. Natl. Acad. Sci. USA, (1988), 85-544.
Almoguera et al., Cell, (1988), 53-549.
Higuchi et al., Nat., (1987), 332-543.
Li et al., Nat., (1988), 335-414.
Higuchi et al., Nuc. Acid. Res., (1988), 1629.
Erlich et al., Natl., (1988), 331-461.
Higuchi et al., Nat., (1988), 332-543.
Li et al., Nat., (1988), 335-414.
Higuchi et al., Nuc. Acid. Res., (1988), 15-7351.
Farr et al., Proc. Natl. Acad. Sci., USA, (1988), 85-1629.
Erlich et al., Nat., (1988), 331-461.
Marx JL, Sci., (1988), 240-1408.
Oste C, Bio. Tech., (1988), 6(2)-162.
Mole et al., Nuc. Acid. Res., (1989),17-3319.
Brown Gregory S.
Jones Theodore
Richards Rodney M.
Snitman David L.
Amgen Inc.
Eggert Joan D.
Levy Ron K.
Odre Kevin M.
Zitomer Stephanie W.
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