Method for reducing carryover contamination in an amplification

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 6, 935 77, 935 78, C12P 1934, C12Q 168

Patent

active

058769765

ABSTRACT:
The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4876187 (1989-10-01), Duck et al.
patent: 5334515 (1994-08-01), Rashtchian et al.
Longo et al., Gen. 93(1990) 125-128.
Syvanen et al., Nucl. Acids Res. 16(23), 11327-11338 (1988).
Vosberg, Hum. Gen., 83(1), 1-1-15 (1989).
Orlandi et al., Proc. Natl. Acad. Sci. USA 86 (1989), 3833-3837.
Wong et al., Nat. (1987), 330-384.
Kwok et al., J. Virol., (1987), 61-1690.
Bos et al, Nat., (1987), 327-293.
Mullis, K. and Faloona, f., Methods in Enzymology, (1987) 155-335.
Ou et al, Sci., (1988), 239-295.
Saiki et al, Sci., (1988), 230-1350.
Engelke et al, Proc. Natl. Acad. Sci. USA, (1988), 85-544.
Almoguera et al., Cell, (1988), 53-549.
Higuchi et al., Nat., (1987), 332-543.
Li et al., Nat., (1988), 335-414.
Higuchi et al., Nuc. Acid. Res., (1988), 1629.
Erlich et al., Natl., (1988), 331-461.
Higuchi et al., Nat., (1988), 332-543.
Li et al., Nat., (1988), 335-414.
Higuchi et al., Nuc. Acid. Res., (1988), 15-7351.
Farr et al., Proc. Natl. Acad. Sci., USA, (1988), 85-1629.
Erlich et al., Nat., (1988), 331-461.
Marx JL, Sci., (1988), 240-1408.
Oste C, Bio. Tech., (1988), 6(2)-162.
Mole et al., Nuc. Acid. Res., (1989),17-3319.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for reducing carryover contamination in an amplification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for reducing carryover contamination in an amplification , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for reducing carryover contamination in an amplification will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-421678

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.