Method for reducing carryover contamination in an amplification

Details Method for reducing carryover contamination in an amplification Method for reducing carryover contamination in an amplification

1995-01-23

2000-03-14

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435810, 435195, 435199, C12P 1934, C12Q 168, C12N 914, C12N 924

Patent

active

060371528

ABSTRACT:
The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused by contaminant amplification product to be reduced or eliminated through the incorporation of at least one modification into the amplification product. The modified amplification product is readily distinguishable from the target sequence in a test sample. Prior to amplifying the target in a new test sample, the sample may be treated to selectively eliminate the contaminant amplification product so that it cannot be amplified in the new sample.

REFERENCES:
patent: 4876187 (1989-10-01), Duck et al.
patent: 5035996 (1991-07-01), Hartley
patent: 5334515 (1994-08-01), Rashtchian et al.
Longo, et al., Gene 93:125-128.

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