Method for recovery of refined .alpha.-galactosidase

Details Method for recovery of refined .alpha.-galactosidase Method for recovery of refined .alpha.-galactosidase

1975-04-30

1976-08-03

Shapiro, Lionel M.

Textiles: fiber preparation

C07G 7028

Patent

active

039727777

ABSTRACT:
A method for the recovery of refined .alpha.-galactosidase is disclosed. This method comprises the steps of bringing an .alpha.-galactosidase-containing liquid into contact with a weakly acidic cation-exchange resin in the presence of a buffer solution for thereby allowing .alpha.-galactosidase to be selectively adsorbed by said resin, desorbing the adsorbed .alpha.-galactosidase from said resin by use of a buffer solution of a type such that at least one of the two factors, concentration and pH, has a higher value than that of the buffer solution used in said adsorption and thereafter recovering the desorbed .alpha.-galactosidase.

REFERENCES:
patent: 3623955 (1971-11-01), Keay
patent: 3846239 (1974-11-01), Delente et al.
Malhotra et al., Biochemische Zeitschrift 340, pp. 565-566 (1964).

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