Method for recovery of nucleic acids

Details Method for recovery of nucleic acids Method for recovery of nucleic acids

2006-06-27

2006-06-27

Naff, David M. (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S440000, C536S025400

Reexamination Certificate

active

07067287

ABSTRACT:
Methods for the recovery of nucleic acids from a nucleic acid-containing material are provided, by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the yield. The methods are composed of a step for promoting the release of nucleic acids from a nucleic acid-containing material, a step for mixing the released nucleic acids with an accelerator substance for the binding of nucleic acids to a solid phase, a step for making the mixture in contact with a solid phase bondable to nucleic acids, a step for isolating the solid phase from a liquid, a step for washing the solid phase with a solution containing a salt, and a step for eluting the nucleic acids from the solid phase. Accordingly, nucleic acids at a suitable purity for genetic tests or gene analyses can be rapidly and easily recovered without the use of hazardous substances.

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