Method for recovery and purification of isoamylase by adsorption

Details Method for recovery and purification of isoamylase by adsorption Method for recovery and purification of isoamylase by adsorption

1995-02-21

1998-09-22

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435200, 210660, C12N 944, C12N 924

Patent

active

058112774

ABSTRACT:
The present invention relates to a method for recovery and purification of isoamylase by raw starch adsorption method. Various raw starches are used to recover the isoamylase produced by a bacterium such as Pseudomonas amyloderamosa. The method comprises using raw starch to adsorb isoamylase under conditions suitable for such adsorption and isolating the adsorbed enzyme. The isoamylase adsorption rate varied with the species of raw starch. Additionally, raw starch can be recycled.

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Fang et al., "Recovery of Isomylase from Pseudomonas amyloderamosa by Adsorption-Elution on Raw Starch." Enzyme Microb. Technol., 16:247-252, Mar. 1994.
Saha et al., "Raw Starch Adsorption-Desorption Purification of a Thermostable .beta.-Amylase from Clostridium thermosulfurogenes." Analytical Biochemistry 175:569-572, Dec. 1988.

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