Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-03-01
2005-03-01
Brumback, Brenda (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Separation or purification
C530S422000, C530S424000
Reexamination Certificate
active
06861505
ABSTRACT:
The present invention relates in providing a process for recovering a soluble protein having a higher recovery rate by suppressing the aggregation of the protein when a denaturing agent is removed from the solubilization treatment solution containing a solubilized protein; and providing a process for removing a denaturing agent from the above solubilization treatment solution in which the aggregation of the protein is suppressed. In a process for recovering a soluble protein comprising the steps of removing a denaturing agent from a solubilization treatment solution prepared by solubilizing insoluble protein aggregates using the denaturing agent, the process of the present invention is characterized by subjecting the solubilization treatment solution to a treatment of removing the denaturing agent in the presence of a dihydric to tetrahydric, polyhydric alcohol at a concentration of 60 to 99% by volume, and thereafter diluting with a diluent solution the treatment solution obtained after the removal treatment. In a process for removing a denaturing agent from a solubilization treatment solution prepared by solubilizing insoluble protein aggregates using the denaturing agent, the process of the present invention is characterized by subjecting the solubilization treatment solution to a treatment of removing the denaturing agent in the presence of a dihydric to tetrahydric, polyhydric alcohol.
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Database Chemabs 'Online!, Chemical Abstracts Service, Columbus, Ohio, USA, Shimamoto, et al, “Efficient Solubilization Of Proteins Overproduced As Inclusion Bodies By Use Of An Extreme Concentration Of Glycerol”, retrieved from STN, Database accession no. 130:232959 HCA, XP-002173883, Abstract, &Tech. Tips Online (1998).
Birch & Stewart Kolasch & Birch, LLP
Brumback Brenda
Gupta Anish
HSP Research Institute, Inc.
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