Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1990-10-09
1992-04-28
Wax, Robert A.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530399, 435 691, C07K 324
Patent
active
051091216
ABSTRACT:
A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed.
The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
REFERENCES:
patent: 3624251 (1971-11-01), Blum et al.
patent: 4857317 (1989-08-01), Wiznendaele et al.
patent: 4988798 (1991-01-01), Blum et al.
Blum Galina
Lee Eun K.
Yang Ren-Der
Farquer Thomas L.
Furman Keith C.
Guffey Wendell Ray
Pitman-Moore Inc.
Wax Robert A.
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