Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-08-26
1994-10-04
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
424 946, 424 9461, 435814, 435815, C12N 916, A61K 3754
Patent
active
053526015
ABSTRACT:
A method for recovering a purified 52,000 dalton fraction of human pancreatic cholesterol esterase is disclosed. The 52,000 dalton fraction of human pancreatic cholesterol esterase is purified by passing a solution having a pH of about 8.2 which contains two or more fractions of human pancreatic cholesterol esterase including the 52,000 dalton fraction over DEAE-cellulose to produce an effluent containing said 52,000 dalton fraction. Since the 52,000 dalton fraction of the esterase does not bind to the DEAE-cellulose whereas the other esterase fractions do bind to the DEAE-cellulose, the 52,000 dalton fraction is then collected in the effluent. The method can be further carried out by passing the purified 52,000 dalton fraction over both hydroxyapatite and heparin-Sepharose to obtain a homogeneous solution of the 52,000 dalton fraction.
REFERENCES:
patent: 4011138 (1977-03-01), Terada et al.
patent: 5017565 (1991-05-01), Lange, III et al.
patent: 5063210 (1991-11-01), Lange, III et al.
patent: 5173408 (1992-12-01), Lange, III et al.
Treadwell et al., in Handbook of Physiology, Section 6, vol. III (1968) pp. 1407-1438.
Brockerhoff et al., in Lipolytic Enzymes, Chp. 5 (Academic Press, 1974) pp. 176-193.
Rudd et al., in Lipases (Elsevier, N.Y., 1984) pp. 185-204.
Calame et al., (1975) Arch. Bioch. Biophys. 168:57-65.
Van den Bosch et al., (1973) Biochem. Biophys. Acta. 296:94-104.
Momsen et al., (1977) Biochem. Biophys. Acta. 486:103-113.
Guy et al., (1981) Eur. J. Biochem. 117:457-460.
Casdorph H. R., in Lipid Pharmocology (Academic Press, 1976) pp. 221-256.
Ayotte and Perlin, (1986) Carbohydrate Res. 145:267-277.
Cardin et al., (1984) Thrombosis Res. 34:541-550.
Julien et al; Biochim. Biophys. Acta 280:215-224 (1972).
Vandermeers et al; Biochim. Biophys. Acta 370:257-268 (1974).
Matsumura et al; J. Biol. Chem. 251(20):6267-6273 (1976).
Brecher et al; Biochim. Biophys. Acta 530:112-123 (1978).
Lombardo et al; Biochim. Biophys. Acta 527:142-149 (1978).
Tuhackova et al; Biochim. Biophys. Acta 617:439-445 (1980).
Tsujita et al; Biochim. Biophys. Acta 715:181-188 (1982).
Durham, III et al; J. Biol. Chem. 259(12):7433-7438 (1984).
Kinnunen Paula M.
Lange, III Louis G.
Spilburg Curtis A.
Lange, III Louis G.
Meller Michael V.
Naff David M.
LandOfFree
Method for recovering purified 52,000 dalton fraction of human p does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for recovering purified 52,000 dalton fraction of human p, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for recovering purified 52,000 dalton fraction of human p will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-580848