Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1995-02-10
1996-02-20
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435814, 435815, 424 946, 424 9461, C12N 916, A61K 3754
Patent
active
054928222
ABSTRACT:
Intestinal cell endogenous heparin mediated absorption of cholesterol or fatty acids in mammals is inhibited through the oral administration of heparin, an active heparin subfraction or heparinase. Suppression of cholesterol esterase-mediated absorption in humans can be inhibited by soluble heparin by two mechanisms, i.e. displacement of the enzyme from the intestinal cell membrane and inhibition of enzymatic activity of the displaced enzyme. A method for recovering a solution of purified human pancreatic cholesterol esterase having a molecular weight of about 100,000 daltons is disclosed. The 100,000 dalton fraction of human pancreatic cholesterol esterase is purified first, by preparing a solution of dialyzed human pancreatic cytosol containing the esterase and other proteins. Next, the solution is passed over a hydroxyapetite column and then eluted to yield all of the molecular weight fractions of the esterase and other proteins in the solution present as a single peak to give a first eluent. Thirdly, the solution is then passed over a gel filtration column and the esterase is collected as a single peak to give a second eluent including the esterase and protein impurities. Next, the second eluent is dialyzed and then it is passed over a chromatography column containing a chromatography material including heparin agarose and heparin-Sepharose, and then finally washing the column and collecting a third eluent which contains the purified esterase.
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Kinnunen Paula M.
Lange, III Louis G.
Spilburg Curtis A.
Lange, III Louis G.
Meller Michael V.
Naff David M.
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