Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2005-01-11
2005-01-11
Russel, Jeffrey Edwin (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C435S183000, C530S344000, C530S412000, C536S025410, C536S128000, C554S022000
Reexamination Certificate
active
06841659
ABSTRACT:
A method for extracting proteins from the intercellular space of plants is provided. The method is applicable to the large scale isolation of many active proteins of interest synthesized by plant cells. The method may be used commercially to recover recombinantly produced proteins from plant hosts thereby making the large scale use of plants as sources for recombinant protein production feasible.
REFERENCES:
patent: 3910822 (1975-10-01), Pentchev et al.
patent: 3972777 (1976-08-01), Yamada et al.
patent: 4104125 (1978-08-01), Takechi et al.
patent: 4885248 (1989-12-01), Ahlquist
patent: 5173410 (1992-12-01), Ahlquist
patent: 5316931 (1994-05-01), Donson et al.
patent: 5466788 (1995-11-01), Ahlquist et al.
patent: 5500360 (1996-03-01), Ahlquist et al.
patent: 5567321 (1996-10-01), Weber et al.
patent: 5589367 (1996-12-01), Donson et al.
patent: 5597569 (1997-01-01), Siegall et al.
patent: 5602242 (1997-02-01), Ahlquist et al.
patent: 5627060 (1997-05-01), Ahlquist et al.
patent: 5837826 (1998-11-01), Flickinger et al.
patent: 5972679 (1999-10-01), Griffith
patent: 6284875 (2001-09-01), Turpen et al.
patent: 6441147 (2002-08-01), Turpen et al.
patent: 6617435 (2003-09-01), Turpen et al.
patent: 2 124 176 (1999-01-01), None
Stenesh. Dictionary of Biochemistry and Molecular Biology, Second Edition, New York: John Wiley & Sons. p. 498, 1989.*
Klement. Method of Obtaining Fluid from the Intercellular Spaces of Foliage and the Fluid's Merit as Substrate for Phytobacterial Pathogens. Phytopathological Notes. 55:1033-1034(1965).*
Parent et al. Detection of pathogenesis-related proteins (PR or b) and of other proteins in the intercellular fluid of hypersensistive plants infected with tobacco mosaic virus. Can. J. Bot. 62:564-569 (1984).*
Trudel et al. Expression of Active Hen Egg Lysozyme in Transgenic Tobacco. Plant Science. 87(1):55-67(1992).*
De Neve et al. Assembly of an antibody . . . Transgenic Research. 1993, vol. 2, pp. 227-237.*
Harris et al. Protein Purification Methods. Oxford: IRL Press. 1989, pp. 9, 10, 62.*
Austin et al. An Overview of a Feasibility Study for the Production of Industrial Enzymes in Transgenic Alfalfa. Annals New York Academy of Science. 721:234-244 (1994).*
De Wilde et al. Intact antigen-binding MAK23 antibody and Fab fragment accumulate in intercellular spaces ofArabidopsis thaliana. Plant Science. 114:223-241 (1996).*
Harris et al. Protein Purification Methods. Oxford: IRL Press. 1989, pp, 9, 10, 62.*
De Wilde et al. Intact antigen-binding MAK23 antibody and Fab fragment accumulate in intercellular spaces ofArabidopsis thaliana. Plant Science. 114:223-241 (1996).*
De Neve et al. “Assembly of an antibody . . . ”. Transgenic Research. 1993, vol. 2, pp. 227-237.*
Harris et al. Protein Purification Methods. Oxford: IRL Press. pp. 9, 10, 62, 1989.*
Stenesh. Dictionary of Biochemistry and Molecular Biology, second edition. New York: John Wiley & Sons. p. 498, 1989.*
Bradford, M., “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding,”Anal. Biochem. 72:248-254 (1976).
Denecke et al., “Protein Secretion in Plant Cells Can Occur via a Default Pathway,”The Plant Cell2:51-59 (1990).
Firek et al., “Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures,”Plant Molecular Biology23:861-870 (1993).
H itz et al., “Two Apoplastic α-Amylases Are Induced in Tobacco by Virus Infection1,”Plant Physiol. 97:651-656 (1991).
Herbers et al., “A Thermostable Xylanase fromClostridium thermocellumExpressed at High Levels in the Apoplast of Transgenic Tobacco Has No Detrimental Effects and is Easily Purified,”Bio/Technology13:63-66 (1995).
Jervis, L. and Pierpoint, W.S., “Purification technologies for plant proteins,”Journal of Biotechnology11:161-198 (1989).
Jones, R. and Robinson, D., “Transley Review No. 17 Protein secretion in plants,”New Phytology111:567-597 (1989).
Kinai et al., “Processing and Secretion of a Virally Encoded Antifungal Toxin in Transgenic Tobacco Plants: Evidence for a Kex2p Pathway in Plants,”The Plant Cell7:677-688 (1995).
Kumagai et al., “Rapid, high-level expression of biologically active α-trichosanthin in transfected plants by an RNA viral vector,”Proc. Natl. Acad. Sci. USA90:427-430 (1993).
Liu et al., “In vivo and in vitro activity of truncated osmotin that is secreted into the extracellular matrix,”Plant Science121:123-131 (1996).
Lowenthal et al., “Production of Interferon-y by Chicken T Cells,”J. Interferon and Cytokine Res. 15:933-938 (1995).
Ma et al., “Generation and Assembly of Secretory Antibodies in Plants,”Science268:716-719 (1995).
Maggio et al., “Large Quantities of Recombinant PR-5 Proteins from the Extracellular Matrix of Tobacco: Rapid Production of Microbial-Recalcitrant Proteins,”Plant Molecular Biology Reporter14(3):249-260 (1996).
Melchers et al., “Extracellular targeting of the vacuolar tobacco proteins AP24, chitinase and β-1,3-glucanase in transgenic plants,”Plant Molecular Biology21:583-593 (1993).
Rathmell, W. and Sequeira, L., “Soluble Peroxidase in Fluid from the Intercellular Spaces of Tobacco Leaves,”Plant Physiol. 53:317-318 (1974).
Regalado, A. and Ricardo, C., “Study of the Intercellular Fluid of HealthyLupinus albusOrgans,”Plant Physiol. 110:227-232 (1996).
Sato et al., “Synthesis and Secretion of Tobacco Neutral PR-5 Protein by Transgenic Tobacco and Yeast,”Biochemical and Biophysical Research Communications211(3):909-913 (1995).
Sijmons et al., “Production of Correctly Processed Human Serum Albumin in Transgenic Plants,”Bio/Technology8:217-221 (1990).
Turpen et al., “Malarial Epitopes Expressed on the Surface of Recombinant Tobacco Mosiac Virus,”Bio/Technology13:53-57 (1995).
Van den Bulcke et al., “Characterization of vacuolar and extracellular β(1,3)-glucanases of tobacco: Evidence for a strictly compartmentalized plant defense system,”Proc. Natl. Acad. Sci. USA86:2673-2677 (1989).
Voss et al., “Reduced virus infectivity inN. tabacumsecreting a TMV-specific full-size antibody,”Molecular Breeding1:39-50 (1995).
Trudel, J., et al. “Expression of Active Hen Egg Lysozyme in Transgenic Tobacco,”Plant Science, 87(1):55-67 (1992).
Written Opinion of corresponding PCT Application (PCT/US99/18161), dated Jun. 21, 2000.
Response to Written Opinion of Corresponding PCT Application (PCT/US99/18161), as submitted on Sep. 20, 2000.
International Preliminary Examination of Corresponding PCT Application (PCT/US99/18161), dated Oct. 18, 2000.
Cameron Terri I.
Garger Stephen J.
Holtz R. Barry
McCulloch Michael J.
Samonek-Potter Michelle L.
Beavers Lucian Wayne
Large Scale Biology Corporation
Robbins John C.
Russel Jeffrey Edwin
Waddey & Patterson
LandOfFree
Method for recovering proteins from the interstitial fluid... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for recovering proteins from the interstitial fluid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for recovering proteins from the interstitial fluid... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3414118