Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Patent
1996-11-01
1998-06-23
Saucier, Sandra E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
530380, 530381, 530382, 530383, 530384, A23J 100, C07K 100, A61K 3514
Patent
active
057707057
ABSTRACT:
Enhanced production of cryoprecipitate is produced by dehydrating an individual unit of plasma prior to a low temperature step used to produce cryoprecipitate. This dehydration is accomplished either by placing a water absorbing material within a blood bag so that plasma occupying the bag will become dehydrated or by placing the water absorbing material within a cartridge so that plasma becomes dehydrated upon flowing through the cartridge. The preferred water-absorbing material is a cross-linked chromatographic gel having pores too small to admit clotting proteins, but large enough to admit water molecules. Suitable gels are made from carbohydrates or polyacrylamide. Carbohydrate gels such as Sephadex.RTM., produced by Pharmacia-Upjohn, are particularly preferred in the present invention. An alternative embodiment of the invention replaces the simple water-absorbing gel with one that also has ion exchange capabilities, such as DEAE (diethy-aminoethyl) Sephadex which has an especial affinity for a number of blood clotting factors collectively known as the prothrombin complex. Following the dehydration step the DEAE Sephadex is eluted to produce prothrombin complex.
REFERENCES:
patent: 3560475 (1971-02-01), Fekete et al.
patent: 3631018 (1971-12-01), Shanbrom et al.
patent: 3682881 (1972-08-01), Fekete et al.
patent: 4069216 (1978-01-01), Shanbrom
patent: 4093608 (1978-06-01), Iga et al.
patent: 4305871 (1981-12-01), Shanbrom
patent: 4555344 (1985-11-01), Cussler
patent: 5696236 (1997-12-01), Omar et al.
Saucier Sandra E.
Shanbrom Technologies LLC
LandOfFree
Method for recovering proteins from plasma using insoluble, wate does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for recovering proteins from plasma using insoluble, wate, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for recovering proteins from plasma using insoluble, wate will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1395505