Method for recovering a purified animal growth hormone or polype

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.

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530412, 530416, 530417, 530418, 530422, 530820, 530825, 530427, 435 68, C07K 328

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048311200

ABSTRACT:
An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor C.sub.I renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator P.sub.L O.sub.L from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the vector is present in the host cell.

REFERENCES:
patent: 4426323 (1984-01-01), Jain
patent: 4512922 (1985-04-01), Jones et al.
patent: 4612367 (1986-09-01), Grinnan et al.
patent: 4656255 (1987-04-01), Seely
patent: 4658021 (1987-04-01), Goeddal et al.
patent: 4677196 (1987-06-01), Rausch et al.
patent: 4693973 (1987-09-01), Buell
patent: 4728609 (1988-08-01), Bhatt et al.
Principles of Biochemistry, Lehninger 1982, p. 177.

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