Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Scleroproteins – e.g. – fibroin – elastin – silk – etc.
Patent
1995-02-03
1998-01-20
Prouty, Rebecca E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Scleroproteins, e.g., fibroin, elastin, silk, etc.
530412, 435 691, A61K 3839
Patent
active
057102520
ABSTRACT:
A method for preparing and purifying collagen-type polypeptides (or biopolymers) has been developed. The polypeptides are prepared by recombinant DNA technology using yeast host cells. Once expressed in the host cell culture, the polypeptide is purified by a series of centrifugations, isoprecipitation, ion exchange (or other chromatography), and diafiltration steps. Treatments with a protease and a calcium salt are also carried out at some point. It is also desirable to diafilter the purified fractions from the last chromatography to exchange ions which make the resulting polypeptide more compatible with its intended use.
REFERENCES:
patent: 3314861 (1967-04-01), Fujii
patent: 4826765 (1989-05-01), Greene et al.
patent: 5114840 (1992-05-01), Tryggvason et al.
patent: 5212074 (1993-05-01), Kiefer et al.
patent: 5217891 (1993-06-01), Brake et al.
patent: 5235041 (1993-08-01), Cappello et al.
patent: 5243038 (1993-09-01), Ferrari et al.
patent: 5496712 (1996-03-01), Cappello et al.
Englard et al., 182 Meth. Enz. 285-300 (Deutscher, ed. 1990).
Kennedy, 182 Meth. Enz. 339-43 (Deutscher, ed. 1990).
Tirrell et al, MRS Bulletin I, XVI (7), pp. 23-28, 1991.
Goldberg et al, Gene, 80, pp. 305-314, 1989.
Futuretech.RTM. Briefing No. 78, Mar. 27, 1989.
Herz Arthur Herman
Weber Shane Crawford
Eastman Kodak Company
Longton Enrique D.
Prouty Rebecca E.
Tucker J. Lanny
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